Figure 6.

Immunohistochemistry analysis of a tau conformational nanobody using human brain samples. Immunohistochemical staining of fixed brain sections from a human brain with a high level of Alzheimer’s disease neuropathological change [(ADNC), NIA-AA criteria (A3, B3, C3)] was performed using WA2.22-Fc fusion protein (left) and zagotenemab (right). WA2.22 and zagotenemab staining was detected using horseradish peroxidase and developed with 3,3’-diaminobenzidine. Nuclei were detected via hematoxylin stain. The scale bars in the main images represent approximately 50 µm, and the scale bars in the insets represent approximately 20 µm.