FIG. 9.

Induction of lytic antigens in infected 293 cells. (A) Detection of EA-D by Western analysis using MAb R3 and a horseradish peroxidase-conjugated secondary antibody. Infected clones of 293 cells were either transfected with pCMV-RZ (17) by the calcium phosphate method (14) with a 20% glycerol shock 5 h later or mock transfected, as indicated. P3HR1 c1 16 cells were induced by diluting a nearly saturated culture into fresh medium containing 3 mM sodium butyrate. Lysates from 200,000 cells of each cell line were analyzed. (B) Detection of EBV Z transactivator by Western analysis using MAb BZ1 as for panel A.