Figure 5. Assessment of ADNKA after S subunit Ab depletion.

(A–D) Sera from individuals with hybrid immunity (PV2; n = 5) were diluted 1:9 and then Abs targeting the indicated domains of S were depleted using magnetic bead–conjugated protein. These depleted sera were then used to measure CD57⁺ NK cell CD107a expression at the indicated dilutions in the presence of SARS-CoV-2–infected A549-ACE2. Four individuals are depicted. (E) The AUC for each sample in A–D was calculated; then, the amount of ADNKA remaining following depletion of Abs targeting each domain in relation to sera containing all Abs, or sera from which all S Abs that had been depleted, was determined (n = 5). Lines bisecting groups in A–D represent mean ± SD.