TABLE 3.

The PCR primers for segmental amplification of chicken mtDNA genes.

S. No.	Gene name	mtDNA position	Primer sequences (5′-3′)	Tm (oC)	Amplification size (bp)
1	D-loop	270-336	F: GAATGGTTACCGGACATAAAC	50	67
			R: CCATTCATAGTTAGGTGACTT	49
2	ND4	11366-11530	F: CCTTGTACCTATTCTAATACTG	47.5	165
			R: CATACTCTTGCCCACAGCC	55.9
3	D-loop	216-436	F: CTCATTTACCCTCCCCATAA	50	221
			R: GTTGCTGATCTCTCGTGAG	51
4	COX1	8050-8341	F: TATTCATCGTCTGAGAAGCCT	53.1	292
			R: TGGTTGGCCATGTGAGATG	55.3
5	ND2	6008-6177	F: ATTAACCGGCTTCATGCCAG	55.7	170
			R: TTATGTGGTTTGATGAGTTGG	50.7
6	rRNA	1996-2157	F: AGGTCAAGGTATAGCCTATGA	50	162
			R: GTATGTACGTGCCTCAGAG	51
7	ND2	5529-5853	F: ATATTAACAATAGCAATCGCAAC	49.7	325
			R: AGGTGAGAATAGTGAGTTGTG	51.8
8	ND4	12476-12796	F: AACACAAACTACGAACGGAT	48	321
			R: TATGAGAAGATGTTCTCGAG	48
9	rRNA	3916-4253	F: TCAACTGCCAAGAACCCCC	57.8	338
			R: GATTGGCTCTTTAATGAATAGT	48.3
10	CYTB	15115-15391	F: CAATACGGCTGACTCATCCA	52	277
			R: CTCAGGCTCACTCTACTAG	51
11	D-loop	1150-1623	F: CACTTAACTCCCCTCACAAG	52	474
			R: TAGCTGGTGCAGATAACATG	50
12	ND5	14683-15016	F: CCTCAATCCTCCTACATACT	50.4	333
			R: ACAGACTGCTAATAGGGAGC	53.8
13	CYTB	14996-15477	F: GCTCCCTATTAGCAGTCTGT	52	482
			R: GATAGTAATACCTGCGATTGC	50
14	D-loop	369-470	F: ACCCATTTGGTTATGCTCGC	52	101
			R: TGAGACTGGTCATGAAGTAC	50
15	tRNA	6512-6625	F: CCCGGCACACTTTAGTGTG	53	114
			R: TGAAGCGTTAGGCTGTAGTC	52