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. 2022 Sep 20;2:87. Originally published 2022 Jul 13. [Version 2] doi: 10.12688/openreseurope.14894.2

Figure 2. Comparison between the three different microwell systems.

Figure 2.

( A) Overview of the plate layout of each culture system and the individual microwell size. The hydrogel microwell is a 24-well plate agarose-based microwell system with 450 microwells per well, and each microwell has a diameter and height of 300 µm and 500 µm, respectively. The AggreWell is a 24-well plate truncated pyramid microwell structure with 1200 microwells per well, and every microwell has a diameter of 400 µm. The Elplasia is thermoformed microwells in a 96-well plate with 79 microwells per well, and each microwell has a diameter of 500 µm and a height of 400 µm. ( B) Comparison in imaging depth in each microwell system from the periphery (0–5 µm from the surface) compared to the core (60 µm from the surface), using a 40×/1.3 NA immersive oil objective. ( C) The images demonstrate the result after F-actin (phalloidin, green) and nuclei (DAPI, blue) staining inside the microwells, where each well was washed three times before imaging.