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. 2023 Aug 9;14:1255316. doi: 10.3389/fimmu.2023.1255316

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Copyright © 2023 Wang, Shi, Zhang, Yuan, Mao and Ma

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tregs depletion inhibited the astrocytes of ferroptosis. Immunofluorescence was used to show distributions of FSP1 (red, E–H) and GFAP (green, A–D) with the merge pictures shown in (I–L). Double labeled immunofluorescence showed the expression of GFAP (green, M–P) and GPX4 (red, Q–T), and the co-localization was shown in (U–X). The flow cytometry image is shown in (Y) and (Z), with a sample size of N=4 and significance level of ***P<0.005, and ****P<0.001. Scale bars for (A–X) were set at 50 μm.