Figure 8.

DT promoted astrocytes ferroptosis by inhibiting the IL-10/GPX4 pathway. The fluorescence intensities of GPX4 was measured for each group in vitro 4h after OGD treatment, as shown in (A–T). OGD caused a significant decrease in GPX4 protein expression levels (E–H). Conversely, treatment with DT decreased GPX4 expression in astrocytes (I–L). Furthermore, adding IL-10Ra, an IL-10 inhibitor, increased the effect of OGD (M–Q), with N = 4 and **P<0.01, ***P<0.005 indicating statistical significance. (A–P) features a scale bar of 50 μm.