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. 2023 Aug 23;9(34):eadg6693. doi: 10.1126/sciadv.adg6693

Fig. 6. MIF secretion enhanced by MYO1B promotes NB cell invasion and metastasis.

Fig. 6.

(A) Evaluation of MIF concentrations by ELISA in unprocessed conditioned medium (CM) from the indicated siRNA-transfected Kelly cells (n = 4). (B and C) Impact of filtration by filters with 100 kDa or 20-nm pore sizes (B) or RIPA + sonication treatment (C) on MIF concentrations in CM samples from Kelly cells (n = 4). (D) Impact of MIF or MYO1B depletion on MIF concentrations in RIPA + sonication–treated CM samples from Kelly cells (n = 3). (E) Colocalization of MIF with MYO1B in Kelly cells was evaluated by IF confocal microscopy. (F and G) Impact of MIF or MYO1B depletion ± recombinant MIF (rMIF) treatment on cell invasion through Matrigel was evaluated by Incucyte (n = 5 to 8). (H and I) Impact of MIF depletion on the extravasation (H) and metastatic capacity (I) of luciferase-expressing Kelly cells was evaluated using the chick embryo CAM metastasis model. The metastasis burden was measured by BLI. Differences between groups were determined by two-tailed unpaired Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.