Fig. 1. eSox17^FNV rapidly and efficiently generates mouse Sox2-positive cells.

(A) Schematic illustration of the neural reprogramming experiment. (B) Whole-well scans (top) of wells from 12-well plate at reprogramming day 20 using a GFP channel; representative phase-contrast (middle) and corresponding Sox2-GFP fluorescence (bottom) images of reprogramming cells in indicated conditions at day 20. Scale bar, 80 μm. (C) Number of Sox2-GFP⁺ colonies in indicated conditions from day 0 to day 20. (D) Counts of Sox2-GFP⁺ colonies at day 20 of reprogramming in indicated 4F conditions. (E) Representative microscope images of miNSC reprogramming with B/K/M/eSox17^FNV from day 0 to day 18 and picked miNSC colonies at passages 1, 2, and 10. Neurorosette-like structures are indicated by white arrows. Scale bar, 160 μm. (F and G) The number of Sox2-GFP⁺ colonies in indicated 3F (F) and 2F/one-factor (G) conditions from day 0 to day 20 of reprogramming. (H) Counts of GFP⁺ colonies at day 20 of reprogramming in indicated 3F and 2F conditions containing eSox17^FNV. (C), (F), and (G) show a representative reprogramming time course performed in three technical replicates as mean ± SEM, and (D) and (H) show data points from three independent experiments done in triplicate, the black bar denotes the mean. Source data are provided in data S1. B, Brn4; K, Klf4; M, c-Myc.