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Titration binding curves of WT and R156-A MBP-NP for F-actin. Sedimentation assays were performed by using 3 μM F-actin and WT (open circles) or mutant R156-A (closed circles) MBP-NP. The amount of bound MBP-NP (expressed as the molar ratio relative to actin) is plotted against the concentration of free MBP-NP.
