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. 2023 Jul 9;16(7):1444–1450. doi: 10.14202/vetworld.2023.1444-1450

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Copyright: © Tan, et al.

Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Figure-1 — Verification of in situ hybridization (ISH) digoxigenin (DIG) probe development by agarose electrophoresis. DIG-labeled probes were successfully developed using porcine circovirus 3 (PCV3) positive samples L43 and L53 as templates. Unlabeled probes were expected to be 69 bp, while corresponding labeled probes were expected to appear larger. The generated probes were used in subsequent ISH staining protocol. Lane 0: DNA ladder (Qiagen^® GelPilot 50 bp ladder). Lane 1: Positive band for unlabeled experimental PCV3 probe from sample L43. Lane 2: Positive band for DIG-labeled experimental PCV3 probe from sample L43. Lane 3: Negative control. Lane 4: Positive band for unlabeled experimental PCV3 probe from sample L53. Lane 5: Positive band for DIG-labeled experimental PCV3 probe for sample L53. Lane 6: Negative control. Lane 7: DNA ladder (Qiagen^® GelPilot 100 bp plus ladder).