Table-1.
The targeting primers used in one-step RT-PCR for FMDV-serotyping.
| Serotype | Primer Designation | Primer sequence (5’-3’) | Ann. Temp. | Amplicon size | References |
|---|---|---|---|---|---|
| All serotypes | 1F | GCCTGGTCTTTCCAGGTCT | 60°C | 328 bp | [26] |
| 1R | CCAGTCCCCTTCTCAGATC | ||||
| (O forward) | O-1C583F | GACGGYGAYGCICTGGTCGT | 60°C | 842 bp | [27] |
| (A forward) | A–1C612F | TAGCGCCGGCAAAGACTTTGA | 60°C | 814 bp | [28] |
| SAT2 forward | SAT2-Egy-F | TGAYCGCAGTACACAYGTYC | 60°C | 666 bp | [29] |
| Reverse primer for (O, A, and SAT2) | EUR–2B52R | GACATGTCCTCCTGCATCTGGTTGAT | --- | --- | [28] |
RT-PCR=Reverse transcription polymerase chain reaction, FMDV=Foot-and-mouth disease virus