FIG. 1.

Examples of KSHV latent, immediate-early, and early genes. The BC-1 cell samples in lanes 1 to 3 were not exposed to a chemical agent that induces the KSHV lytic cycle. The samples in lanes 4 to 14 were treated with 3 mM n-butyrate for the time periods indicated (in hours) at the top of each lane. The cell samples in lanes 1 and 10 were treated with 0.5 mM PAA for 30 h to inhibit viral DNA synthesis. The samples in lanes 2 and 11 to 14 received cycloheximide in the indicated dose for 13 or 20 h to inhibit protein synthesis. The same total cellular RNA samples were hybridized with probes specific for vFLIP, a latent product; for Rta, an immediate-early product; and vIL-6, an early product. To control for RNA loading, the Northern blots were probed for cellular H1 RNA, a component of RNase P (3).