FIG. 4.

Kinetics of early lytic gene expression of KSHV in the BC-1 PEL cell line. BC-1 cells were treated with 3 mM n-butyrate for the time indicated before total RNA was extracted. In lane 8, an RNA sample was prepared from cells that were treated with 0.5 mM PAA and 3 mM n-butyrate from time zero to 30 h. In lane 9, an RNA sample was prepared from cells that were treated with 3 mM n-butyrate and 100 μg of cycloheximide per ml from time zero to 13 h. The same RNA preparation was used for the hybridization with different probes, as indicated on the left of the blots. (A) Expression of the cellular β-actin gene and the viral early lytic genes, vMIP II gene, ORF K3, ORF K5, ORF K8, vIL-6, and PAN RNA. (B) Expression of cellular H1 RNA and viral delayed early and late lytic genes, viral thymidylate synthase (vTS), vMIP I, G-protein coupled receptor (vGCR), ORF K12, vBcl2, and sVCA gene.