Table 4.

Summary of combinatorial mutation effects. (A) Ph-dependent binding scores and amino acid residues of the template sequence and top 10 sequences proposed by MTS. (B) Ph-dependent binding score and amino acid residue for best single mutations at each position included in the initial training data.

sequence name	amino acid residues							pH-dependent binding score
	VH30	VH54	VH55	VL26	VL52	VL54	VL95
template	S	S	G	S	S	L	S	0.451
MTS-1	S	K	G	S	K	R	I	1.147
MTS-2	S	K	K	F	K	R	I	1.143
MTS-3	S	K	K	F	K	L	I	1.116
MTS-4	R	S	G	S	K	R	I	1.107
MTS-5	S	S	K	S	K	R	I	1.100
MTS-6	S	K	K	F	S	R	I	1.091
MTS-7	S	K	G	S	K	L	I	1.089
MTS-8	S	K	K	F	K	R	S	1.075
MTS-9	R	K	G	S	K	R	I	1.073
MTS-10	S	K	K	F	S	L	I	1.057
amino acid residue
	VH30	VH54	VH55	VL26	VL52	VL54	VL95
Single best AA	R	K	K	F	K	R	I
pH-dependent binding score	0.706	0.628	0.770	0.852	0.516	0.597	0.651