Table 5.

The results of Biacore sensorgram among the sequence with top Ph-binding score on MTS, the best with single mutation, and the template sequence.

sequence name	pH 7.4			pH 6.0			KD ratio (pH 6.0/pH 7.4)
	ka (M − 1s − 1)	kd (s − 1)	KD (M)	ka (M − 1s − 1)	kd (s − 1)	KD (M)
template	(1.0 ± 0.015) ×105	(2.2 ± 0.015) ×10−3	(2.1 ± 0.015) ×10−8	(7.4 ± 4.3) ×103	(6.1 ± 2.3) ×10−2	(8.8 ± 1.5) ×10−6	421
COSMO-1	(1.1 ± 0.0058) ×105	(6.2 ± 0.025) ×10−4	(5.5 ± 0.023) ×10−9	(8.9 ± 0.10) ×104	(1.3 ± 0.015) ×10−2	(1.4 ± 0.035) ×10−7	26
MTS-1	(1.6 ± 0.029) ×105	(2.9 ± 0.017) ×10−4	(1.8 ± 0.038) ×10−9	(1.0 ± 0.046) ×105	(6.5 ± 0.067) ×10−3	(6.5 ± 0.22) ×10−8	37