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. 2023 Aug 23;12:RP85380. doi: 10.7554/eLife.85380

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© 2023, Wang, Jin et al

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Figure 5. — (A) Construction of the Foxc2^f/-;Ddx4^Cre/+ mice. (B) The testes size of the Foxc2^f/-;Ddx4^Cre/+ mice. Scale bar, 1 mm; M, month. (C) Body weight and testis weight of the Foxc2^f/-;Ddx4^Cre/+ mice at different age (n=5). M, month; values, mean ± s.e.m.; p-values were obtained using two-tailed t-tests (ns >0.05, ****p-value <0.0001). (D) Hematoxylin and eosin (HE) staining of the testis and epididymis. Scale bar, 50 μm; M, month. (E) Estimation of degenerative tubules and sperm counts in cauda epididymis of the Foxc2^f/+ and Foxc2^f/-;Ddx4^Cre/+ mice with age (n=5). Values, mean ± s.e.m.; p-values were obtained using two-tailed t-tests (**p-value <0.01, ****p-value <0.0001). (F) Immunostainings for DAPI (blue), ZBTB16 (green), FOXC2 (magenta), and DDX4 (white) in the seminiferous tubules of the Foxc2^f/+ and Foxc2^f/-;Ddx4^Cre/+ mice. Scale bar, 50 μm. (G) Estimation of ZBTB16⁺ uSPG number in the Foxc2^f/+ and Foxc2^f/-;Ddx4^Cre/+ mice with age (n=5). Values, mean ± s.e.m.; p-values were obtained using two-tailed t-tests (ns >0.05, ***p-value <0.001, ****p-value <0.0001). (H) Quantitative RT-PCR analysis of the uSPG and germ cell markers expressed in the testis of the Foxc2^f/+ and Foxc2^f/-;Ddx4^Cre/+ mice (n=3). M, month; values, mean ± s.e.m.; p-values were obtained using two-tailed t-tests (**p-value <0.01, ***p-value <0.001, ****p-value <0.0001).

Figure 5—figure supplement 1. — (A) Construction of the Foxc2^f/-;Ddx4^Cre/+ mice. (B) The testes size of the Foxc2^f/-;Ddx4^Cre/+ mice. Scale bar, 1 mm; M, month. (C) Body weight and testis weight of the Foxc2^f/-;Ddx4^Cre/+ mice at different age (n=5). M, month; values, mean ± s.e.m.; p-values were obtained using two-tailed t-tests (ns >0.05, ****p-value <0.0001). (D) Hematoxylin and eosin (HE) staining of the testis and epididymis. Scale bar, 50 μm; M, month. (E) Estimation of degenerative tubules and sperm counts in cauda epididymis of the Foxc2^f/+ and Foxc2^f/-;Ddx4^Cre/+ mice with age (n=5). Values, mean ± s.e.m.; p-values were obtained using two-tailed t-tests (**p-value <0.01, ****p-value <0.0001). (F) Immunostainings for DAPI (blue), ZBTB16 (green), FOXC2 (magenta), and DDX4 (white) in the seminiferous tubules of the Foxc2^f/+ and Foxc2^f/-;Ddx4^Cre/+ mice. Scale bar, 50 μm. (G) Estimation of ZBTB16⁺ uSPG number in the Foxc2^f/+ and Foxc2^f/-;Ddx4^Cre/+ mice with age (n=5). Values, mean ± s.e.m.; p-values were obtained using two-tailed t-tests (ns >0.05, ***p-value <0.001, ****p-value <0.0001). (H) Quantitative RT-PCR analysis of the uSPG and germ cell markers expressed in the testis of the Foxc2^f/+ and Foxc2^f/-;Ddx4^Cre/+ mice (n=3). M, month; values, mean ± s.e.m.; p-values were obtained using two-tailed t-tests (**p-value <0.01, ***p-value <0.001, ****p-value <0.0001).