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Negatively stained EM images of Gag Δp6 and Gag particles assembled in vitro. (A) Gag Δp6 particles assembled with yeast tRNA; (B) Gag Δp6 particles assembled with an in vitro transcript of HIV-1 packaging sequence (∼1 kb); (C) Gag particles assembled at 4°C with E. coli rRNA; (D) Gag Δp6 particles assembled with MS2 bacteriophage RNA (∼3.5 kb); arrows indicate tails protruding from particles. All panels were negatively stained with 2% uranyl acetate. Scale bars = 50 nm.
