Extended Data Fig. 6. NRF2 regulates carbon consumption and nucleotide synthesis.
a) The indicated metabolites were quantified by the YSI bioanalyzer. The production was determined by calculating the difference between blank and spent medium (6 hr incubation of T cells collected in the late phase/72 hr) (n = 3 experimental replicates, P < 0.001 and P = 0.0027 for lactate and glutamate production). b) OCR (in the late phase/72 hr) was determined by the Seahorse (n = 12 experimental replicates for basal and n = 9 experimental replicates for maximal OCR, P < 0.001 and P < 0.001 for basal and maximal OCR respectively). Data from a-b are representative of 3 independent experiments. c) The level of the indicated mRNA in cells collected 72 hr after activation was determined by qPCR and depicted by heatmap (log 10-fold change) (n = 3 experimental replicates). Data are representative of 2 independent experiments. Error bars represent mean ± SD. *P < 0.05, **P < 0.01, *** P < 0.001. Statistical differences were determined by unpaired Two-tail Student’s t-test (a and b).