Fig. 7. Asn restriction enhances T cell-mediated antitumor responses in vivo.
a–h, Schematic diagrams of animal experiments (a, c, e and g). Kaplan–Meier survival curves and tumor growth of mice bearing a B16-gp100 tumor (b) (n = 10 mice per group; P = 0.0054 and P < 0.0001 for the left and right panels, respectively). Data are representative of three independent experiments. Kaplan–Meier survival curves and tumor growth of mice bearing a LAN-1 neuroblastoma tumor (d) (n = 8 mice per group; P < 0.0001 and P < 0.0001 for the left and right panels, respectively). Data are representative of two independent experiments. Kaplan–Meier survival curves and tumor growth of mice bearing a B16F10 melanoma tumor (f) (n = 5 mice per group; P = 0.0019 and P < 0.0001 for the left and right panels, respectively). Data are representative of three independent experiments. Kaplan–Meier survival curves and tumor growth of mice bearing a CMT Keap-1 KO lung tumor (h) (n = 5 mice per group; P = 0.0085 and P < 0.0001 for the left and right panels, respectively). Data are representative of two independent experiments. Error bars represent mean ± s.e.m. (b, d, f and h). **P < 0.01; ***P < 0.001. Statistical differences were determined by two-way ANOVA for tumor growth curve and log-rank test for animal survival. i, The conceptual model of ATF4-dependent and NRF2-dependent stress signaling response, optimized carbon assimilation, and robust effector function on CD8+ T cells.