Extended Data Fig. 9. Effect of DZNeP on intestinal proliferation in WT mice and on stem cell markers in APC and APC KRAS mice.
Quantification of IHC for BrdU incorporation in the (A,B) small intestine and (C,D) colon of WT mice treated with vehicle or DZNeP (5 mg/kg). (n = 3 mice per experimental arm; panels A,C: each dot represents the number of BrdU positive cells counted per half crypt. Line represents mean; panels B,D: Mean ± SEM, each dot represents the average number of BrdU positive cells per mouse. (E) Representative images and quantification of ISH for Olfm4 and Lgr5 expression in the small intestine of APC KRAS mice (n = 4) treated with vehicle or DZNeP (5 mg/kg). Scale bars: 50 μm. [Mean ± SEM). Each dot represents the average % positive area per crypt for each mouse. Asterisks refer to p-values obtained from 1-tailed Mann-Whitney tests (*: p = 0.0143). Images for Lgr5 were processed using imageJ to show ISH staining in red. (F) Volcano plot showing transcriptomic changes in small intestinal tissues of APC mice treated with vehicle (n = 4) or DZNeP (5 mg/kg; n = 5) for 4 days. (G) Plots showing changes in expression of stem cell markers in small intestinal tissues of APC mice treated with vehicle (n = 4) or DZNeP (5 mg/kg; n = 5) for 4 days. (Mean ± SD). Each dot represents values obtained for an individual mouse.