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. 2023 Aug 14;5(8):1303–1318. doi: 10.1038/s42255-023-00857-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (A) Multivariate analysis of REIMS data showing segmentation when focusing analysis on the full acquired mass range (m/z = 50-1500), or (B) low mass range (m/z: 50-250). Colour coding: yellow = WT; green = KRAS; cyan = APC; pink = APC KRAS; blue = APC KRAS PTEN. Each dot corresponds to a single mass spectrum acquired using the REIMS forceps. Data acquired from WT (n = 3), KRAS (n = 4) APC (n = 11), APC KRAS (n = 4), and APC KRAS PTEN (n = 5) mice. (C) Clustering plot of REIMS data (m/z: 600-1500) showing genotype-dependent clustering. ♂/♀ indicate male/female dominant APC clusters. (D) t-SNE of DESI-MSI data acquired from longitudinal sections of the rolled up small intestine of WT, KRAS, APC, and APC KRAS mice focusing analysis on ions within high mass range m/z: 700-1200, or (E) low mass range m/z: 50-250 (n = 4 per genotype, each image is a rolled small intestine from one individual mouse). (F) Targeted LC-MS analysis of polar extracts of colonic tissues from KRAS, APC, and APC KRAS mice compared with WT mice. Heatmap constructed based on fold change between the averages of each experimental group (n = 3).

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