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. 2023 Aug 14;5(8):1303–1318. doi: 10.1038/s42255-023-00857-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — (A) H&E of distal colon tissue with an Apc-deficient tumour [same H&E as shown in Fig. 2B and Extended Data Fig. 3A; N. Adj.: normal adjacent tissue; S: stroma; T: tumour tissue]. Tissues analysed for n = 4 animals; n = 1 shown. Scale bar: 1 mm. (B) DESI-MSI showed decreased abundance of an ion with m/z 215.03 in the tumour epithelial compartment of APC tumours. [N. Adj.: normal adjacent tissue; S: stroma; T: tumour tissue]. Database search (https://hmdb.ca) suggested that this could be assigned as glucose [M+Cl]^-. A standard solution of glucose was mixed with chlorinated dopamine to obtain chlorinated glucose for comparison. (C) DESI tandem mass spectrometry showed that the fragmentation of the ion of interest produced ions matched to the fragmentation pattern of the chlorinated standard, further supporting the ID of this metabolite of interest. All highlighted m/z of fragments and precursor ions on the mass spectra are present in both standard and sample.