Extended Data Fig. 4. Comparative untargeted metabolomics of APC and APC KRAS tumours by DESI-MSI and LC-MS.
(A) Volcano plot showing metabolic differences between tumour epithelial regions of APC (n = 2 mice) and APC KRAS (n = 2 mice) colon tumours as analysed by DESI-MSI. Red dots: FC ≥ 1.5 and significant after Benjamini-Hochberg FDR correction (q = 0.1). (B) Representative images of glutamine abundance in APC and APC KRAS colon tumours as analysed by DESI-MSI. Tissues analysed for n = 4 APC mice and n = 3 APC KRAS mice; n = 1 shown. (C) Volcano plot showing metabolic differences between bulk tumour tissue extracts of APC (n = 5 mice) and APC KRAS (n = 8 mice as analysed by untargeted LC-MS. Red dots: FC ≥ 1.5 and significant after Benjamini-Hochberg FDR correction (q = 0.1). If a more stringent FDR was used (for example q = 0.05) no metabolites pass significance for the LC/MS data.