TABLE 1.
Distribution of species of particles produced by T3D and tsA279 at permissive and restrictive temperaturesa
| Clone | Temp (°C) | Time (h) | Total no. of particlesb | Total no. of particles/mlc | Proportion of particle (% [SEM])d
|
|||
|---|---|---|---|---|---|---|---|---|
| Virion | Top component | Outer shell | Core | |||||
| T3D | 32 | 36 | 1,773 | 1.7 × 108 | 85.7 (4.3) | 6.5 (0.7) | 2.7 (0.8) | 5.2 (3.0) |
| 72 | 24,682 | 2.9 × 109 | 78.6 (<0.1) | 15.4 (0.3) | 5.9 (0.3) | 0.1 (<0.1) | ||
| 40 | 36 | 1,632 | 1.3 × 108 | 64.1 (2.4) | 22.4 (2.8) | 9.1 (1.8) | 1.7 (0.3) | |
| 72 | 4,904 | 4.4 × 108 | 56.1 (8.5) | 22.5 (5.4) | 9.9 (0.7) | 11.5 (4.0) | ||
| tsA279 | 32 | 36 | 12,546 | 1.3 × 109 | 76.9 (3.8) | 19.0 (3.2) | 2.1 (0.6) | 1.9 (1.1) |
| 72 | 31,659 | 1.3 × 109 | 79.0 (1.0) | 18.8 (1.8) | 2.0 (1.8) | 0.2 (0.2) | ||
| 40 | 36 | 3,401 | 3.2 × 107 | 60.6 (2.7) | 19.6 (3.9) | 1.0 (0.4) | 18.8 (5.9) | |
| 72 | 1,209 | 3.6 × 107 | 50.2 (7.5) | 18.6 (7.3) | 0.7 (0.7) | 30.5 (15.1) | ||
a
Calculated from three to four separate experiments.
b
Total number of viral and subviral particles counted.
c
Total number of particles in the cell lysates, calculated as previously described (38).
d
Proportion of indicated particle type, expressed as the percentage of the total number of particles produced, with the standard error of the mean of replicate experiments shown in parentheses.