FIG. 2.

Properties of p6-deleted Gag polyproteins. (A) COS-1 cells were transfected with the indicated DNAs and labeled as described in Materials and Methods. Molecular mass standards (in kilodaltons) are indicated to the left. (B) Distribution of particle size in rate-zonal gradients. COS-1 cells were transfected with the indicated DNAs, and after 48 h were labeled with [³⁵S]methionine for 5 h. After the labeling period, particle sizes were analyzed as described in Materials and Methods. Radiolabeled RSV from infected TEF cells was added to the gradients to provide an internal control. Arrows indicate the direction of sedimentation.