FIG. 2.
Reporter gene expression mediated by recombinant EBOV nucleocapsid proteins. HeLa cells were infected with MVA-T7; transfected with 500 ng of pT/NPEBO, 500 ng of pT/VP35EBO, 100 ng of pT/VP30EBO, and/or 1 μg of pT/LEBO; and subsequently transfected with RNA 3E-5E. At 3 days p.i., cells were lysed. CAT activity was determined, and acetylated products were separated by thin-layer chromatography. DNA transfection was performed with different combinations of nucleocapsid protein genes as indicated. NP, pT/NPEBO; 35, pT/VP35EBO; 30, pT/VP30EBO; L, pT/LEBO.