Table 2.
Detection of rAAV6 capsid protein in CB-CD34+ HSPCs at 24 and 48 h post-genome-editing treatment
| Sample | HR% | NHEJ% | VP3 |
|---|---|---|---|
| Producer cell supernatant | N/A | N/A | detected |
| Untreated | N/A | N/A | not detecteda |
| RNP 24 h | N/A | 90.57 | not detecteda |
| RNP/rAAV6 24 h | 72.01 | 22.75 | not detecteda |
| RNP 48 h | N/A | 90.57 | not detecteda |
| RNP/rAAV6 48 h | 73.49 | 21.27 | not detecteda |
RNP, RNP/electroporation; RNP/rAAV6, RNP/electroporation/rAAV6.
Levels were lower than the reliable detection threshold, which was equivalent to 2.00E4 vg/cell.