TABLE 1.
Primer sequences used for directed mutagenesis of the HCV-1b 5′UTRa
| Mutant | Primer binding site location (nt) | Primer sequence |
|---|---|---|
| A172G-U227C | 240–218, 213–235 | 5′-GGGGGCACGCCCAGATCTCCAGG (primer 1), 5′-CAATGCCTGGAGATCTGGGCGTG (primer 2) |
| G229A-C238U | 252–227, 222–247 | 5′-TAGCAGTCTCGCGGAGGCACGCCTAA (primer 1), 5′-GAGATTTAGGCGTGCCTCCGCGAGAC (primer 2) |
| C232G-G235C | 245–224, 219–241 | 5′-CTCGCGGGGGGACCCCCAAATC (primer 1), 5′-CTGGAGATTTGGGGGTCCCCCCG (primer 2) |
| G235A-C232U | 245–216, 219–241 | 5′-CTCGCGGGGGTACACCCAAATC (primer 1), 5′-CTGGAGATTTGGGTGTACCCCCG (primer 2) |
| G229C-C238G | 250–222, 217–245 | 5′-GCAGTCTCGCGGCGGCACGCCGAAATCTC (primer 1), 5′-GCCTGGAGATTTCGGCGTGCCGCCGCGAG (primer 2) |
a
Positions of mutations are underlined in the mutant name and shown in boldface in the primer sequence. Template plasmid DNAs are shown in boldface in the mutant name.