REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, peptides, and recombinant proteins | ||
Wheat germ agglutinin (WGA), Alexa FluorTM 594 conjugate | Thermo Fisher Scientific | W1126 |
Oregon Green® 488 BAPTA-1 dextran, 10,000 MW | Thermo Fisher Scientific | O6798 |
Dextran, Cascade BlueTM, 10,000 MW | Thermo Fisher Scientific | D1976 |
Dextran, Alexa FluorTM 488, 10,000 MW | Thermo Fisher Scientific | D22910 |
Dextran, Alexa FluorTM 568, 10,000 MW | Thermo Fisher Scientific | D22912 |
Dextran, Alexa FluorTM 594, 10,000 MW | Thermo Fisher Scientific | D22913 |
CellTrace™ Calcein Violet, AM | Thermo Fisher Scientific | C34858 |
CellTrace™ Calcein Green, AM | Thermo Fisher Scientific | C34852 |
CellTrace™ Calcein Red-Orange, AM | Thermo Fisher Scientific | C34851 |
Fluo-4, AM, cell permeant | Thermo Fisher Scientific | F14201 |
Dimethylsulfoxid (DMSO) | Merck (before Sigma-Aldrich) | Cas#67-68-5; |
Pluoronic F-127 (10% in water) | Biotium | Cas#9003-11-6 |
MK-571, sodium salt | Enzo (before Alexis Biochemicals) | Cas#115104-28-4 |
Tricaine (MS-222) | TCI chemicals | Cas#886-86-2 |
L-Amino acids (analytical standard) L-Alanine, L-Arginine Hydrochloride, L-Cysteine hydrochloride, Glycine, L-Histidine hydrochloride, L-Isoleucine, L-Leucine, L-Lysine hydrochloride, L-Methionine, L-Phenylalanine, L-Serine, L-Threonine, L-Tryptophan, L-Valine |
Merck (before Sigma-Aldrich) | LAA21 Cas#56-41-7, Cas#1119-34-2, Cas#52-89-1, Cas#56-40-6, Cas#5934-29-2, Cas#73-32-5, Cas#61-90-5, Cas#657-27-2, Cas#63-68-3, Cas#63-91-2, Cas#56-45-1, Cas#72-19-5, Cas#73-22-3, Cas#72-18-4 |
Bile acids Taurocholic acid, Glycocholic acid, Cholic acid, Deoxycholic acid |
Merck (before Sigma-Aldrich) | Cas#345909-26-4, Cas#1192657-83-2, Cas#81-25-4, Cas#83-44-3 |
Alcohols, Aldehydes, Ketones α-Terpineol, β-ionone, β-phenylethyl alcohol, γ-phenylpropyl alcohol, Citral |
Merck (before Sigma-Aldrich) | Cas#98-55-5, Cas#14901-07-6, Cas#60-12-8, Cas#122-97-4, Cas#5392-40-5 |
CleanCap® Cre mRNA (5moU) | TriLink Biotechnologies | Cas#L-7211 |
Experimental models: Organisms/strains | ||
Xla.Tg(tubb2b:Katushka; cryga:Venus) | The European Xenopus Resource Center | RRID:EXRC_0202 |
Xla.Tg(tubb2b:GCaMP6s; Rno.elas:GFP) | The National Xenopus Resource | RRID:NXR_0107 |
Xla.Tg(CMV:Brainbow-1.0H) | Livet et al.,63 | RRID:NXR_0001 |
Software and algorithms | ||
CaImAn | Giovannuci et al., 2019 | RRID:SCR_021152 |
Fiji | Schindelin et al.64 | RRID:SCR_002285 |
Vaa3D | Peng et al., 2010 | RRID:SCR_002609 |
Inkscape | Inkscape project | RRID:SCR_014479 |
Other | ||
Cyclo II peristaltic pump | Roth | EP76.1 |
MilliManifoldTM 16 to 1 channel | ALA Scientific Innstruments | MilliManifoldTM 16 |
8-channel, gravity perfusion system with pinch valve controllers | ALA Scientific Innstruments | VC3-8PG |
Borosilicate microcapillaries | Warner Instruments | 2BF150-86-10 |
Micropipette Puller P-1000 | Sutter Instrument | RRID:SCR_021042 |
Micropipette Beveler | World Precision Instruments | Micropipette Beveler 48000 |
Femto Jet express microinjector | Eppendorf AG | Femto Jet express |
Electroporator | NPI Electronics | ELP-01D |
Axoporator® 800A | Axon Instruments/Molecular Devices | Axoporator 800A |
Micromanipulator for injection setup | Scientifica | PatchStar |
Fluorescence stereomicroscopes | Olympus | BX51WI; SZX16 |
Fluorescent light source | Andor Technology-Oxford Instruments | AMH-200-F6S |
Fluorescent light source | Excelitas technologies (before: Lumen Dynamics) | X-Cite 120Q |
Multiphoton microscope | Nikon | A1R MP; RRID:SCR_020319 |