Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 17;299(8):105056. doi: 10.1016/j.jbc.2023.105056

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fluorescence anisotropy measurement of MANT-ATP nucleotide in WT and D67N OaPAC as a function of protein concentration at fixed (1 μM) MANT-ATP concentration. As the concentration of protein is increasing, the population of bound nucleotide will also increase resulting in higher fluorescence anisotropy. The obtained binding affinity from the fit was 2.2 ± 0.4 μM in WT and 7.7 ± 1.6 μM in D67N. MANT, 2'-(or-3')-O-(N-methylanthraniloyl) adenosine 5′-triphosphate, trisodium salt; OaPAC, photoactivated adenylate cyclase from Oscillatoria acuminata.