REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-B220 FITC, clone RA3-6B2	BD	Cat.# 553088; RRID:AB_394618
anti-CD5 BB700, clone 53-7.3	BD	Cat.# 742083; RRID:AB_2871364
anti-CD19 BV421, clone 1D3	BD	Cat.# 562701; RRID:AB_2737731
anti-CD21 APC, clone 7G6	BD	Cat.# 561770; RRID:AB_10892818
anti-CD43 PE, clone S7	BD	Cat.# 553271; RRID:AB_394748
anti-CD23 PE-Cy7, clone B3B4	BioLegend	Cat.# 101614; RRID:AB_2103036
anti-CD16/32	BD	Cat.# 553142; RRID:AB_394657
anti-IgD PE, clone 11 -26c.2a	BD	Cat.# 558597; RRID:AB_647211
anti-CD19 PE-CF594, clone 1D3	BD	Cat.# 562329; RRID:AB_11154580
anti-IgM APC, clone RMM-1	BioLegend	Cat.# 406509; RRID:AB_315059
anti-CD43 APC-Cy7, clone 1B11	Biolegend	Cat.# 121220; RRID:AB_2194192
anti-CD5 PerCP-Cy5.5, clone 53-7.3	ebioscience	Cat.# 45-0051-82; RRID:AB_914334
biotinylated anti-CD9, clone KMC8	BD	Cat.# 558749; RRID:AB_397103
anti-CD63 PE, clone NVG-2	BD	Cat.# 564222; RRID:AB_2738678
biotinylated anti-CD81, clone Eat2	BD	Cat.# 559518; RRID:AB_397259
anti-IgM HRP	SouthernBiotech	Cat.# 1020-05; RRID:AB_2794201
anti-IgG2a HRP	SouthernBiotech	Cat.# 1080-05; RRID:AB_2734756
anti-CD81 BV421, clone Eat2	BD	Cat.# 740060; RRID:AB_2739825
rat IgG2a FITC isotype control, clone R35-95	BD	Cat.# 554688; RRID:AB_10053774
rat IgG2a APC isotype control, clone RTK2758	Biolegend	Cat.# 400512; RRID:AB_2814702
anti-CD4 PE-Cy5, clone GK1.5	ebioscience	Cat.# 15-0041-82; RRID:AB_468695
anti-IgG AF488	ThermoFisher Scientific	Cat.# A11055; RRID:AB_2534102
Chemicals, peptides, and recombinant proteins
Lipopolysaccharide	Sigma	Cat.# L6511
PtC liposomes FITC	Dr. Aaron Kantor, Kantor Bioscience Consulting
streptavidin PE	ebioscience	Cat.# 12-4317-87
RBC lysis buffer	Lonza	Cat.# 10-548E
LIVE/DEAD fixable cell stain	Invitrogen	Cat.# L34966
RIPA lysis buffer	ThermoFisher Scientific	Cat.# 89900
Halt protease inhibitor cocktail	ThermoFisher Scientific	Cat.# 1862209
Halt phosphatase inhibitor cocktail	ThermoFisher Scientific	Cat.# 1862495
Triton X-100	Sigma	Cat.# X100
Tween 20	Sigma	Cat.# 9416
Laemmli SDS sample buffer	Bio-Rad	Cat.# 1610747
streptavidin HRP	Pierce	Cat.# 21130
purified mouse IgM	BD	Cat.# 557275
wheat germ agglutinin AF488	Invitrogen	Cat.# W11261
phalloidin rhodamine	ThermoFisher Scientific	Cat.# R415
Prolong Gold/DAPI	ThermoFisher Scientific	Cat.# P10144
Critical commercial assays
Miltenyi gentleMACS Octo dissociator	Miltenyi	Cat.# 130-095-937
Miltenyi pan B cell isolation kit II	Miltenyi	Cat.# 130-104-443
bicinchoninic acid (BCA) protein assay kit	Pierce	Cat# 23227
4-15% tris-glycine gels	Bio-Rad	Cat# 4568086
Aldehyde sulfate beads (4 μm); Aldehyde sulfate beads (5μm)	ThermoFisher Scientific	Cat.# A37304; Cat.# A37306
imperial protein stain	ThermoFisher Scientific	Cat# 24615
anti-mouse IgM ELISA kit	Bethyl	Cat.# E90-101
round glass coverslips, #1.5, 12mm	Thomas Scientific	Cat.# 1217N79
Immun-Blot PVDF membrane	Bio-Rad	Cat# 1620177
SuperSignal West Pico PLUS ECL reagents	ThermoFisher Scientific	Cat# 34580
Lionheart FX automated live cell imager	Biotek
AR1+ confocal microscope	Nikon
Fortessa flow cytometer	BD
Influx cell sorter	BD
Experimental models: Cell lines
WEHI-231 mouse B cell lymphoma	frozen stock
S9.6 hybridoma	American Type Culture Collection (ATCC)	Cat.# HB-8730; lot 63476317
CH-12 mouse B cell lymphoma	frozen stock
HELA human cervical cancer	frozen stock
Experimental models: Organisms/strains
C57BL/6 mouse	The Jackson Laboratory	https://www.jax.org
Ighm knock-out mice (B6; 129S4-Ighmtm1che/J)	Dr. Kishore Alugupalli
Software and algorithms
Flowjo, v10.6.2	BD	https://www.flowjo.com
Image J, version 1.51	National Institutes of Health	https://imagej.nih.gov/ij/download.html
Prism, version 8.1.1	GraphPad	https://www.graphpad.com/scientific-software/prism/
Photoshop 2018, 2021	Adobe	https://www.adobe.com/products/photoshop.html
Image Lab	Bio-Rad	https://www.bio-rad.com/en-us/product/image-lab-software?ID=KRE6P5E8Z
Gen5 software (Lionheart microscope)	Biotek
NIS-Elements C (Nikon confocal microscope)	Nikon