FIG. 6.

Kinetics of reverse transcription and processivity the HERV-K RT enzyme. (Left) HERV-K RT samples of clones 7.1, 10.1, and 10.9 were used in the standard poly(rA)-oligo(dT) assay, and the length of the cDNA products of the HERV-K RT enzymes was analyzed on a denaturing sequencing gel (lanes 1 to 3) and compared with the products of the HIV-1 and AMV RT enzymes (lanes 5 and 6). The GST protein was used as negative control (lane 4). (Right) Standard RT assay with clones 7.1, 10.1, and 10.9. Samples were analyzed for dTTP incorporation at several times up to 4 h. The background values obtained for the GST control protein were subtracted.