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Preparation of colony selection tubes
(A) Gather a 250 μL pipette tip.
(B) Cut the wide end of a pipette tip so the tip fits within a closed 1.5 mL flip-cap tube.
(C) Place the shortened pipette tip into a previously autoclaved 1.5 mL tube.
(D) Close the tube and sterilize it by autoclaving at 121°C at 15 Psi for 20 min.
