Table 4.

PCR reaction master mix

Reagent	Amount
Bullseye Red TAQ Polymerase Mastermix	6 μL × # of samples
27F Primer: 5′-AGAGTTTGATCMTGGCTCAG-3′	0.25 μL × # of samples
1525R Primer: 5′-AAGGAGGTGATCCAGCC-3′	0.25 μL × # of samples
PCR grade water	1.5 μL × # of samples