REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Chemicals, peptides, and recombinant proteins | ||
Lactobacilli MRS broth (De Mann Rosa Sharpe) | BD Biosciences | Cat# DF0881-17-5 |
Tryptic soy broth | BD Bacto | Cat# DF0370-17-3 |
Cooked meat broth | Fisher BD | Cat# 226730 |
Reinforced clostridial broth | Fisher Scientific | Cat# OXCM0149B |
Brain heart infusion broth | Fisher Scientific | Cat# CM1135B |
Chocolate agar | Fisher Scientific | Cat# R01300 |
Bacteroides Bile Esculin agar | Fisher Scientific | Cat# NC0998155 |
Defibrinated Sheep Blood | Fisher Scientific | Cat# 50-863-753 |
Agar | Fisher BD | Cat# DF0140-01-0 |
ExoSAP-IT™ PCR Product Cleanup Reagent | Applied Biosystems | Cat# 78201.1.ML |
NP-40 (IGEPAL® CA-630) | Sigma-Aldrich | Cat# 18896 |
Bullseye Red TAQ DNA Polymerase Mastermix∗ | MIDSCI | Cat# BE180303 |
Software and algorithms | ||
BioRender | BioRender | N/A |
Other | ||
16s primer: 27F, 5′-AGAGTTTGATCMTGGCTCAG-3′ | N/A | N/A |
16s primer: 1525R, 5′-AAGGAGGTGATCCAGCC-3′ | N/A | N/A |
∗Cannot be substituted. Can be purchased at: https://midsci.com/item/ASPCRREAG3/PREMIUM-Bullseye-Taq-DNA-Polymerase-Master-Mix/.