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Fluorograph of sialyltransferase reactions and PNGase F digestions using asialofetuin glycoprotein acceptor. Triton X-100 soluble lysates were prepared from tissue culture cells infected with VACV WR, MYXV Lu, or MYXV Lu(lacZ+/MST3N−) and reacted with CMP-[3H]Sia and asialofetuin, with the addition of rat liver sialyltransferase ST6Gal-I or N-glycosidase (PNGase F) as indicated.
