Cytokine gene expression of cIEC after 24 h of invasion. Data are represented as expression relative to the challenged control. The color of the bars defines the category of each compound: red for anticoccidials, green for NIC and yellow for EO. Data are reported as mean with SEM (n = 6). Different letters indicate significant differences among treatments (one-way ANOVA with Tukey's multiple comparisons for normally distributed data and Kruskal–Wallis with Dunn's multiple comparisons for non-normal data), asterisks represent significant differences compared to ET+ (one-way ANOVA with Dunnett's multiple comparisons for normally distributed data and Kruskal–Wallis with Dunn's multiple comparisons for non-normal data; P ≤ 0.05). Abbreviations: AMP, amprolium; BEO, basil essential oil; CAR, carvacrol; DDS, diallyl-disulfide; ET+, Challenged control; GAR, garlic oil; LNL, linalool; OEO, oregano essential oil; SAL, salinomycin; TEO, thyme essential oil; THY, thymol.