Table 1.
List of all primers used for standard quantification and cytokine gene expression.
| Gene | Primer sequence (5′→3′) | Product length (bp) | Accession N. | |
|---|---|---|---|---|
| Cytokines | IL1β | F: TGCCTGCAGAAGAAGCCTCG | 137 | NM_204524.1 |
| R: CTCCGCAGCAGTTTGGTCAT | ||||
| IL6 | F: GCAGGACGAGATGTGCAAGA | 84 | NM_204628.1 | |
| R: ACCTTGGGCAGGTTGAGGTT | ||||
| IL8 | F: AGCTGCTCTGTCGCAAGGTA | 124 | NM_205498.1 | |
| R: GCTTGGCGTCAGCTTCACATC | ||||
| IL10 | F: GTCACCGCTTCTTCACCTGC | 84 | NM_001004414.2 | |
| R: TCCCGTTCTCATCCATCTTCTCG | ||||
| Housekeeping | RPLP0 | F: TTGGGCATCACCACAAAGATT | 83 | NM_204987 |
| R: CCCACTTTGTCTCCGGTCTTAA | ||||
| GAPDH | F: ACTGTCAAGGCTGAGAACGG | 86 | NM_204305 | |
| R: CATTTGATGTTGCTGGGGTC | ||||
| E. tenella DNA marker | ITS1 | F: TGGAGGGGATTATGAGAGGA | 147 | AF026388.1 |
| R: CAAGCAGCATGTAACGGAGA |