Table 3.
Invasion efficiencies of all the treatments at 2 and 24 h obtained by counts of extracellular sporozoites.
| MDBK |
CIEC |
|||
|---|---|---|---|---|
| Treatment | 2 hpi (%) | 24 hpi (%) | 2 hpi (%) | 24 hpi (%) |
| ET+ | 59.66 ± 2.20 a | 100.00 ± 1.34 a | 76.76 ± 1.38 a | 100.00 ± 0.82 a |
| AMP 5 ppm | 37.93 ± 4.34 bc* | 63.13 ± 4.18 ab* | 65.11 ± 1.73 ab | 78.24 ± 1.58 ab |
| SAL 5 ppm | 40.58 ± 1.78 b* | 54.11 ± 2.06 bc* | 46.93 ± 7.09 bc* | 72.98 ± 1.01 ac |
| CAR 20 ppm | 24.59 ± 3.30 bd* | 56.83 ± 4.43 bc* | 59.24 ± 1.83 ac | 69.60 ± 2.19 bc* |
| DDS 20 ppm | 10.88 ± 3.42 d* | 48.81 ± 4.18 bc* | 44.73 ± 3.24 bc* | 62.35 ± 1.64 c* |
| LNL 20 ppm | 26.53 ± 3.85 bd* | 63.40 ± 2.78 ac* | 57.86 ± 4.56 ac | 72.71 ± 1.55 ac |
| THY 20 ppm | 25.11 ± 3.61 bd* | 68.19 ± 2.02 ac* | 63.39 ± 1.91 ac | 73.40 ± 1.64 ac |
| BEO 40 ppm | 25.23 ± 6.21 bd* | 38.90 ± 2.25 bc* | 37.10 ± 6.31 bc* | 63.64 ± 3.01 bc* |
| GAR 40 ppm | 10.27 ± 3.26 cd* | 33.22 ± 4.45 b* | 29.73 ± 3.36 c* | 52.83 ± 3.29 c* |
| OEO 40 ppm | 18.48 ± 4.58 cd* | 42.10 ± 4.70 bc* | 47.42 ± 5.46 bc* | 68.06 ± 2.07 bc* |
| TEO 40 ppm | 30.70 ± 3.58 bd* | 63.43 ± 3.03 bc* | 56.76 ± 4.24 ac | 71.01 ± 1.06 ac* |
AMP, amprolium; BEO, basil essential oil; CAR, carvacrol; DDS, diallyl-disulfide; ET+, Challenged control; GAR, garlic oil; LNL, linalool; OEO, oregano essential oil; SAL, salinomycin; TEO, thyme essential oil; THY, thymol.
Invasion assays were performed on MDBK and cIEC. Data are represented as a percentage on the respective challenged control at 24 hpi. Data are reported as mean with SEM (n = 6). Data were not normally distributed and were analyzed with Kruskal–Wallis test with Dunn's multiple comparisons. Different letters represent significant differences within the same column (same timepoint and same cell model) (P ≤ 0.05). The asterisks indicate significant differences with the ET+ group (P ≤ 0.05).