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. Author manuscript; available in PMC: 2023 Aug 24.
Published in final edited form as: Cell Rep. 2023 Jul 3;42(7):112732. doi: 10.1016/j.celrep.2023.112732

Figure 4. Single-cell fate mapping of monocyte differentiation in PDA.

Figure 4.

(A) t-distributed stochastic neighbor embedding (t-SNE) plots of intratumoral immune cells isolated on days 3 and 7 ± αCD4 post tumor (n = 4 orthotopic KPC2a tumors per group). See also Figure S7.

(B) t-SNE plots of only monocytes and macrophage clusters.

(C) Heatmap showing normalized cluster-specific gene expression. See also Figure S8A.

(D) Normalized CITE-seq protein in t-SNE plot.

(E) Gene set enrichment analysis of canonical pro-inflammatory and anti-inflammatory genes split by monocyte/macrophage cluster defined in (B).

(F) t-SNE plot showing Tomato+ and Tomato− cells from days 3 and 7 untreated, and proportion of each monocyte/macrophage cluster among Tomato+ and Tomato− myeloid cells from clusters defined in (B). See also Figure S8B.

(G) t-SNE plot and proportion of each monocyte/macrophage cluster at days 3 and 7. See also Figure S8C.