KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| anti-mouse CD45 (clone 30-F11) | BD Bioscience | Cat#: 566168; RRID: AB_2739565 |
| anti-mouse Ly6C (clone HK1.4) | Biolegend | Cat#: 128031; AB_2562177 |
| anti-mouse CD11b (clone M1/70) | Biolegend | Cat#: 101237; RRID: AB_11126744 |
| anti-mouse F4/80 (clone BM8) | Biolegend | Cat#: 123149; RRID: AB_2564589 |
| anti-mouse Ly6G (clone 1A8) | Biolegend | Cat#: 127645; RRID: AB_2566317 |
| anti-mouse CD206 (clone C068C2) | Biolegend | Cat#: 141715; AB_2561991 |
| anti-mouse PD-L1 (clone 10F.9G2) | Biolegend | Cat#: 124313; RRID: AB_10639934 |
| anti-mouse FRβ (clone 10F.9G2) | Biolegend | Cat#: 153305; RRID: AB_2721312 |
| anti-mouse Arg1 (clone A1exF5) | ThermoFisher | Cat#: 25369782: RRID: AB_2734841 |
| anti-mouse I-A/I-E (clone M5/114.15.2) | Biolegend | Cat#: 107651; RRID: AB_2616728 |
| anti-mouse Ki67 (clone 16A6) | Biolegend | Cat#: 652420; RRID: AB_2564285 |
| anti-mouse CD3 (clone 17A2) | BD Bioscience | Cat#: 740530: RRID:AB_2740239 |
| anti-mouse CD8 (clone 53–6.7) | Biolegend | Cat#: 100759; RRID: AB_2563510 |
| anti-mouse CD44 (clone IM7) | Tonbo Bioscience | Cat#: 65-0041; RRID:AB_2621847 |
| anti-mouse CD4 (clone RM4-5) | ThermoFisher | Cat#: 56004282; RRID: AB_494000 |
| anti-mouse Klrg1 (clone 2F1) | eBioscience | Cat#: 25589382; RRID: AB_1518768 |
| anti-mouse Foxp3 (clone FJK-16s) | eBioscience | Cat#: 48577382; RRID: AB_1518812 |
| anti-mouse T-bet (clone 4B10) | Biolegend | Cat#: 644810; RRID: AB_2200542 |
| anti-mouse Stat1 (clone D1K9Y) | Cell Signaling | Cat#: 80916S |
| anti-mouse IFNγ (clone XMG1.2) | Invitrogen | Cat#: 24731141 |
| anti-mouse CD19 (clone 1D3) | Biolegend | Cat#: 152403; RRID: AB_2629812 |
| anti-mouse CD115 (clone AFS98) | Biolegend | Cat#:135525: RRID: AB_2566461 |
| anti-mouse Lyve1 (clone ALY7) | ThermoFisher | Cat#: 14-0443-82 |
| anti-human CD11b (clone ICRF44) | Biolegend | Cat#: 301310; RRID:AB_314162 |
| anti-human CD11c (clone 3.9) | eBioscience | Cat#: 25016642 |
| anti-human CD64 (clone 10.1) | Biolegend | Cat#: 305036; RRID: AB_2650834 |
| anti-human CD14 (clone 61D3) | ThermoFisher | Cat#: 11014942; RRID:AB_10597597 |
| anti-human CD15 (clone HI98) | BD Bioscience | Cat#: 564232; RRID: AB_2738686 |
| anti-human FRβ (clone 94b) | Biolegend | Cat#: 391703; RRID: AB_2721335 |
| anti-human HLA-DR (clone L243) | BD Bioscience | Cat#: 552764; RRID: AB_394453 |
| anti-human PD-L1 (clone 29E.2A3) | Biolegend | Cat#: 329722; RRID: AB_2565764 |
| anti-human CD163 (clone GHI/61) | Biolegend | Cat#: 333632; RRID: AB_2728288 |
| InVivoMAb anti-mouse CD4 (clone GK1.5) | BioXcell | Cat#:BE0003-1; RRID: AB_1107636 |
| InVivoMAb anti-mouse CD8 (clone 2.43) | BioXcell | Cat#:BE0061; RRID: AB_1125541 |
| InVivoMAb anti-mouse CD40L (clone MR1) | BioXcell | Cat#:BE0017-1; RRID: AB_1107601 |
| TotalSeq™-A0190 anti-mouse CD274 | Biolegend | Cat#:153604; RRID: AB_2783125 |
| TotalSeq™-A0564 anti-mouse FRβ | Biolegend | Cat#:153307; RRID: AB_2800690 |
| TotalSeq™-A0117 anti-mouse I-A/I-E | Biolegend | Cat#: 107653; RRID: AB_2750505 |
|
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| Biological samples | ||
|
| ||
| Patient PDA Samples | University of Minnesota BioNet | N/A |
|
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| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| PFA 4% | VWR International | Cat#: 9713.1000 |
| Tamoxifen | Sigma | Cat#: T5648 |
| DMEM medium | Life Technologies | Cat#: 21875091 |
| Red blood cell lysis buffer | BD Bioscience | Cat#: 555899 |
| Fetal bovine serum | ThermoFisher | Cat#: 12350273 |
| Amphotericin B | GIBCO | Cat#: 15290018 |
| Penicillin/streptomycin | GIBCO | Cat#: 10378016 |
| Dextrose | ThermoFisher | Cat#: D15-500 |
| EDTA | ThermoFisher | Cat#: 15575020 |
| L-Glutamate | ThermoFisher | Cat#: 25030081 |
| β-mercaptoethanol | GIBCO | Cat#: M6250 |
| Ghost viability dye BV540 | Tonbo | Cat#: 13-0879-T100 |
| Golgiplug | BD Bioscience | Cat#: 555029 |
| Collagenase IV | Sigma Aldrich | Cat#: V900893 |
|
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| Critical commercial assays | ||
|
| ||
| Foxp3 fixation and permeabilization kit | Tonbo | Cat#: TNB-0607-KIT |
| CD4+ T Cell Isolation Kit, mouse | Miltenyi | Cat# 130-092-916 |
| Cell Stimulation Cocktail (500X) | eBioscience | Cat#: 00-4970-03 |
|
| ||
| Deposited data | ||
|
| ||
| Mouse scRNAseq | GEO | GSE233068 |
| Mouse Bulkseq | GEO | GSE233068 |
|
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| Experimental models: Cell lines | ||
|
| ||
| KPC2a cells | Burrack et. al.30 | N/A |
| KPC OVA cells | Burrack et. al.30 | N/A |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| C57BL/6J mice | Jackson Labs | 000664 |
| Ifngr1−/− | Jackson Labs | 003288 |
| Tnfrsf1a−/− | Jackson Labs | 003242 |
| Cd40−/− | Jackson Labs | 002928 |
| CX3CR1CreER (B6.129P2(C)-Cx3cr1tm2.1 (cre/ERT2)Jung/J) | Jackson Labs | 020940 |
| CCR2creERT2 [C57BL/6NTac-Ccr2tm2982 (T2A-Cre7ESR1-T2A-mKate2)] | Croxford et. al.76 | N/A |
| R26-tdTomato (B6.Cg-Gt(ROSA)26Sortm9 (CAG-tdTomato)Hze/J). | Jackson Labs | 007909 |
| CCR2-CreER-GFP (C57BL/6-Ccr2em1 (icre/ERT2)Peng/J) x I-AB-flox (B6.129X1-H2-Ab1tm1Koni/J) | This report | N/A |
| Sm1xRag1−/− | Srinivasan et. al.36 | N/A |
| Rag1−/− (B6.Cg-Rag2tm1.1Cgn/J) | Jackson Labs | 008309 |
| OTII (B6.Cg-Tg(TcraTcrb)425Cbn/J) | Jackson Labs | 004194 |
|
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| Software and algorithms | ||
|
| ||
| FlowJo version 10 | FlowJo | N/A |
| FACS Diva | BD | N/A |
| GraphPad | Prism | N/A |
|
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| Other | ||
|
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| Matrigel | Discovery Labware | 08-774-552 |
| 4.0 Sutures | Ethicon | 1611G |
| 5.0 Sutures | Ethicon | 8580H |