FIG. 2.

Allele-specific suppression of the CT67GA mutant splicing defect by expression of compensatory U11 snRNA. (A) Potential base pairing interaction between the NRS and U11 snRNA. The NRS CT67GA mutation and the U11 snRNA compensatory mutations (AG56CT and AA45TT) at the 5′ end of U11 are shown. (B) RT-PCR of RNA from transfected 293 cells was performed as described in the legend to Fig. 1. Lanes 3 to 5 are reactions from the cells transfected with the NRS CT67GA mutant. Indicated below is cotransfection with an empty vector (−), or the AA45TT (45) or AG56CT (56) U11 snRNA expression plasmid. The arrowhead indicates the spliced band restored by the compensatory AG56CT U11 construct.