Table 2.
Primers and sgRNAs used in this study.
| Primers | Sequences (5′-3′) | Usage |
|---|---|---|
| 3663Up-F61 | GCCACCTGACGTCTAGATCTCGAGCAGATCCTCGATCGCG | Construction of pCM-△3663 and PCR verification of YA3663 |
| 3663Up-R58 | TCCGAGGAGACGCGGACCCGGGGACTTTTCGCGAAACAATCC | Construction of pCM-△3663 |
| 3663Down-F63 | ATTGTTTCGCGAAAAGTCCCCGGGTCCGCGTCTCCTCG | Construction of pCM-△3663 |
| 3663Down-R61 | CTGGATCCTCTAGTGAGCTCCGCGATCTACGGGCTGGC | Construction of pCM-△3663 and PCR verification of YA3663 |
| 3664Up-F59 | GCCACCTGACGTCTAGATCTGAGAATCGTCGCGAACGGC | Construction of pCM-△3664 and PCR verification of YA3664 |
| 3664Up-R60 | ACCGCCTGAGTTGATTCGACCACTCACCCTCCTCGTCCTC | Construction of pCM-△3664 |
| 3664Down-F58 | GAGGACGAGGAGGGTGAGTGGTCGAATCAACTCAGGCGGT | Construction of pCM-△3664 |
| 3664Down-R60 | CGCTCGAGCTGCAGCATATGGGCAAGGTCAAGGATGCGC | Construction of pCM-△3664 and PCR verification of YA3664 |
| 3652up-F58 | GCCACCTGACGTCTAGATCTCCGGCTTCCTCTCGATCAAC | Construction of pCM-△3652 |
| 3652up-R62 | GTCTCGAACTCTCGAAGGTCCGCGTTTCCTCCATTGCGCT | Construction of pCM-△3652 |
| 3652down-F55 | AGCGCAATGGAGGAAACGCGGACCTTCGAGAGTTCGAGAC | Construction of pCM-△3652 |
| 3652down-R57 | CTGGATCCTCTAGTGAGCTCTGCTCACCTCGAAGTTCAGA | Construction of pCM-△3652 |
| 3652-tF | AGATCCGCTTCACCGCCGA | PCR verification of strain YA3652 |
| 3652-tR | TGACAGGGACGGGCTGAAGC | PCR verification of strain YA3652 |
| 4598-Up-F | AAAGTGCCACCTGACGTCTAGATCTACGAGATGGTCGAACATCGCGT | Construction of pCM-△4598 |
| 4598-Up-R | TGCCTATATGAAGGCCATCCCTCGTATCACCGGTCAAGCGTGTCTCGAA | Construction of pCM-△4598 |
| 4598-Down-F | TTCTTCGAGACACGCTTGACCGGTGATACGAGGGATGGCCTTCATATA | Construction of pCM-△4598 |
| 4598-Down-R | TCGGCTGGATCCTCTAGTGAGCTCTCGATACCGCCTCGACCTATT | Construction of pCM-△4598 |
| 4598-tF | GGGATTGAACGGGTTTTCGC | PCR verification of YA4598 |
| 4598-tR | GGTGCCCGTGATGTGCCTGA | PCR verification of YA4598 |
| Com-R | CTATATTTTCTAGGCTTTGATTG | Construction of plasmid pAI1815 and pAI1816 |
| 1815-NT766-F | TCAAAGCCTAGAAAATATAGTGATGGACTTTCTCGCTGAGGTTTTAGAGCTAGAAATAG | Construction of plasmid pAI1815 |
| 1816-NT802-F | TCAAAGCCTAGAAAATATAGAGGAGAAGGGGGAATTTGCCGTTTTAGAGCTAGAAATAG | Construction of plasmid pAI1816 |
| 80-15F63 | ACCATGATTACGCCAAGCTTATGAGCGCCGCGCTTCAAAC | Construction of pCM-exp1815 and pCM-exp1815-1816 |
| 80-15R70 | ACGGGATTCTGTGAGGATCCTCATCGGCCGGCTCCGGCGG | Construction of pCM-exp1815 |
| 80-16F69 | ACCATGATTACGCCAAGCTTATGAGCGCCACCGCCACCCC | Construction of pCM-exp1816 |
| 80-16R63 | AGCTCGGTACCCGGGGATCCTCACAGAATCCCGTGGCGCA | Construction of pCM-exp1816 and pCM-exp1815-1816 |
| 80-3663F-57 | ACCATGATTACGCCAAGCTTATGACACTCACCCTCCTCG | Construction of pCM-exp3663 |
| 80-3663R-58 | AGCTCGGTACCCGGGGATCCTCAACCCGGTTCCTGCC | Construction of pCM-exp3663 |
| 80-3664-F60 | ACCATGATTACGCCAAGCTTATGGCGCGGGGCAAAC | Construction of pCM-exp3664 |
| 80-3664-R60 | AGCTCGGTACCCGGGGATCCTCATCGGGTCCGCGTCTC | Construction of pCM-exp3664 |