PCR Cycling Conditions
| Step | Temperature | Time | Cycles |
|---|---|---|---|
| Activation 1 | 50°C | 2 min | 1 |
| Activation 2 | 95°C | 2 min | 1 |
| Denaturation | 95°C | 15 s | 40 |
| Annealing | 60°C | 15 s | |
| Extension | 72°C | 1 min | |
| Hold | 4°C | Forever | N/A |
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