Figure 3.

Silencing of KRAS by EGFR_apt-3WJ-siKRAS^G12C pRNA nanoparticles suppressed KRAS activity, cell growth, and metastatic capabilities of NSCLC cells

(A) KRAS activity was determined by KRAS activation ELISA assay on H2030 and H2122 cells. (B) Effects of KRAS silencing by pRNA nanoparticles on cell growth was determined by IncuCyte cell proliferation assay (cell number reflects percent confluency). (C) Quantification of transwell migration and invasion assays (top panels) and calculated invasion index (bottom panels). Error bars represent SD. Significance (A and C) was calculated using Student’s t test; significance (B) was calculated using one-way ANOVA analysis followed by Tukey’s post-hoc test: ∗p < 0.05, ∗∗p < 0.001.