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. 2023 Aug 10;14:1174140. doi: 10.3389/fimmu.2023.1174140

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Copyright © 2023 Shukla, Rao, Meena, Giorgianni, Lee, Raju, Shashikanth, Shekhar, Beranova, Balazs, Tigyi, Gosain and Rao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HD5 administered at 24 hours after irradiation modulates altered gut microbiota composition. At 24 hours after sham treatment (Sham) or irradiation (IR), mice were fed a liquid diet with vehicle (Veh-Sham & Veh-IR) or HD5 (HD5-Sham & HD5-IR). After additional 24 hours, the microbiota composition in colonic flushing was analyzed by 16S rRNA-sequencing and metagenomics. (A) The relative abundance of different phyla of bacteria. Data are derived from pooling all values within the group. The experiment was repeated once with similar results. (B) The Shannon Index was used to quantify α-diversity. (C) Principal coordinate analysis (PCoA) based on Bray-Curtis dissimilarity analysis was performed to determine β-diversity. (D-G) Relative abundance of Enterobacteriaceae (D), E. coli (E), Lactobacillus (F), and Akkermansia (G) in different groups.