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. 2023 Aug 10;14:1174140. doi: 10.3389/fimmu.2023.1174140

Figure 5.

Figure 5

HD5 feeding at 24 hours after irradiation mitigates colonic mucosal injury. At 24 hours after sham treatment (Sham) or irradiation (IR) mice were fed a liquid diet with vehicle (Veh-Sham & Veh-IR) or HD5 (HD5-Sham & HD5-IR). After additional 24 hours, TJ and AJ integrity, mucosal permeability, and mucosal inflammatory responses were analyzed. (A, B) TJ integrity was assessed by immunofluorescence staining of colon cryosections for occludin and ZO-1 (green, occludin; red, ZO-1; blue, nucleus) and confocal microscopy. ZO-1 fluorescence density values are presented in panel (B). (C, D) AJ integrity was assessed by staining colon sections for E-cadherin and β-catenin. β-catenin fluorescence density values are presented in panel (D). (E, F) Mucosal permeability in vivo was evaluated in the colon (E) and ileum (F) by measuring the vascular-to-luminal flux of FITC-inulin. Values are mean ± sem (n = 6); **p<0.01, ***p<0.001, and ****p<0.0001 for significant difference between the indicated groups; “ns” = not significant. (G-L) At 48 hours after irradiation (24 hours after start of HD5 treatment), total RNA preparations from the colon were analyzed for IL-1β (G), IL-6 (H), TNFα (I), Mcp1 (J), Cxcl1 (K), and Cxcl2 (L). Values are mean ± sem (n = 6); *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001 for significant difference between the indicated groups; “ns”, not significant.