Figure 3.

acs expression is dynamic after the ingestion of SmDb11 bacteria

(A and B) acs dynamic expression was revealed by in situ hybridization using the RNAscope technique on time-matched uninfected and SmDb11 infected midguts at 8 and 12 h. The acs iso KI mutant is used as a negative control to probe the specificity of the signal. Scale bars 50μm.

(C) acs is expressed in progenitor cells that are identified through their basal position and small size of the nuclei (arrowheads). Scale bar 50μm.

(D and E) acs was knocked down in the enterocytes (NP > acs RNAi iso) or in the ISCs (esg> acs RNAi iso) and flies were infected with SmDb11 (D) or exposed to sucrose (E). % epithelium thickness categories were assessed at 3h and 16h (chi-square, ∗∗∗∗ = p < 0.0001, comparisons at 16h between knocked down flies and corresponding controls crossed with w¹¹¹⁸iso). Qualitative quantification of intestines according to their epithelial thickness: Thin-red, Semithin-yellow, Thick-blue (n = 24–30).