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. 2023 Jul 27;26(9):107490. doi: 10.1016/j.isci.2023.107490

Figure 4.

Figure 4

ACS localizes to the basal side of the enterocytes in the midgut epithelium and is required for the retrograde transport

(A) Confocal pictures of dissected midguts from NP>UAS-acs-GFP flies exposed to sucrose or to SmDb11 for 3h and 6h. Blue:DNA; Red:actin; Green:ACS-GFP; n = 30.

(B) Quantification of free amino acids in the hemolymph of NP > ctrl and NP > acs RNAi 3h after sucrose exposure or SmDb11 infection. Bars represent the mean and the error bars display Standard Deviation of the data (one-way ANOVA, ∗∗∗ = p < 0.001, n = 3 × 20 hemolymphs).

(C) Flies were injected with 50 μM AHA and exposed to regular food, SmDb11 (infected) or water (starved). Midguts dissected 6h later and fluorescence from the incorporated amino acid was assessed by staining with an alkyne probe and measured using ImageJ. The median bars represent the mean while the error bars display Standard Deviation of the data(one-way ANOVA, ∗∗∗∗ = p < 0.0001, n = 16–24). Stainings shown in Figure S2B.